Antibody-antigen interactions in blood typing for paper diagnostics and surface plasmon resonance analysis

Paper-based analytical devices (PADs) can provide a quick, inexpensive and simple detection method for medical diagnostics. The aim of this research is to better understand the antibody-antigen interactions of non-ABO and non-RhD phenotypes in order to develop a robust test which can uniformly detect all blood types using paper diagnostics. However, methods quantifying these antibody-antigen interactions are limited. Surface plasmon resonance (SPR) can quantify interactions between biomolecules. In this thesis a fully regenerable, multi-functional platform for quantitative blood group phenotyping via SPR detection was achieved for the first time by covalently immobilizing an antibody, anti-human IgG, to the chip surface.