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A Single Molecule Localization and In situ Crosslinking Study of the Beta-barrel Assembly Machinery in Escherichia coli

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posted on 2019-03-27, 21:11 authored by SACHITH DILSHAN GUNASINGHE
In order to sense changes in, respond to, and exploit their environmental niches, bacteria rely on structures assembled into their outer membrane. A set of bacterial proteins collectively known as the Beta-barrel Assembly Machinery is solely responsible for catalysing the biogenesis of this membrane. By employing genetic code expansion tools and advanced molecular imaging techniques, the Super-resolution microscopy, we interrogated the interactome and membrane spatial representation of these proteins. Our findings present a missing piece of the mechanistic puzzle on how these proteins catalyse the events involving the propagation of the outer membrane.


Principal supervisor

Trevor Lithgow

Year of Award


Department, School or Centre


Campus location



Doctor of Philosophy

Degree Type



Faculty of Medicine Nursing and Health Sciences