L177 Sachith Dilshan Gunasinghe_Doctoral thesis_2018_Redacted.pdf (8.39 MB)
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A Single Molecule Localization and In situ Crosslinking Study of the Beta-barrel Assembly Machinery in Escherichia coli

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thesis
posted on 27.03.2019, 21:11 by SACHITH DILSHAN GUNASINGHE
In order to sense changes in, respond to, and exploit their environmental niches, bacteria rely on structures assembled into their outer membrane. A set of bacterial proteins collectively known as the Beta-barrel Assembly Machinery is solely responsible for catalysing the biogenesis of this membrane. By employing genetic code expansion tools and advanced molecular imaging techniques, the Super-resolution microscopy, we interrogated the interactome and membrane spatial representation of these proteins. Our findings present a missing piece of the mechanistic puzzle on how these proteins catalyse the events involving the propagation of the outer membrane.

History

Principal supervisor

Trevor Lithgow

Year of Award

2018

Department, School or Centre

Microbiology

Campus location

Australia

Course

Doctor of Philosophy

Degree Type

DOCTORATE

Faculty

Faculty of Medicine Nursing and Health Sciences

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