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72 files

RNAseq from P4 murine whole kidneys with Polycystic Kidney Disease

Version 2 2023-11-11, 00:30
Version 1 2023-11-11, 00:23
dataset
posted on 2023-11-11, 00:30 authored by Denny CottleDenny Cottle, Ian SmythIan Smyth, Ming Shen Tham

Snap frozen Postnatal day4 murine kidney tissues were crushed using micropestles in RLT Buffer and RNA was extracted from these tissues using QiaShredders followed by Qiagen RNAeasy Mini Kits according to manufacturer’s protocol. RNA concentration and integrity were determined by Aligent bioanalyzer. cDNA library preparation was carried out using NEB Next Ultra Directional RNA Library Prep Kit for Illumina RNA sequencing (E7420S) and sequenced on an Illumina HiSeqX10 machine in 150 bp paired end format. Sequence data was processed with Skewer adaptor trimmer and mapped with HiSat2 to the Mus musculus GRCm38_v90 genome assembly. The resulting Bam files are provided here.


Samples were from Control Cre (Hoxb7-cre) mice, Aurka flox/flox; Hoxb7-cre (Aurka Knockout Out -AKO) mice, Inpp5e flox/flox; Hoxb7-cre (Inpp5e Knockout Out -IKO) mice, Pkd1 flox/flox; Hoxb7-cre (Pkd1 Knockout Out -PKO) mice, Inpp5e flox/flox; Aurka flox/flox; Hoxb7-cre (Inpp5e and Aurka double Knockout Out -dKO-I) mice, and Pkd1 flox/flox; Aurka flox/flox; Hoxb7-cre (Pkd1 and Aurka double Knockout Out -dKO-P) mice.


Hoxb7-cre mediated deletion of floxed alleles specifically in the kidney collecting ducts.

Inpp5e KO results in Polycystic Kidney Disease related to Joubert Syndrome.

Pkd1 KO results in Autosomal Dominant Polycystic Kidney disease.

Aurka KO had no phenotype and appeared normal.

Hoxb7-cre control animals had no phenotype and appeared normal.

Both Inpp5e and Aurka, and Pkd1 and Aurka double knockout mice exhibited a rescue in disease.


Sample labels abbreviated to groups as bolded above.


Funding

Australian National Health and Medical Research (NHMRC) APP1046174 Australian National Health and Medical Research (NHMRC) APP1182539 PKD Foundation Senior Research Fellowship of the NHMRC APP1106516 Monash International PhD student scholarship Australian Government Research Training Program (RTP) Scholarship

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