Metadata file for files in this repository item Original data for: Physiological trait variation among indigenous and invasive species: higher basal tolerance but no difference in plasticity at the assemblage level Laura M. Phillips, Ian Aitkenhead, Charlene Janion-Scheepers, Catherine K. King, Melodie A. McGeoch, Uffe N. Nielsen, Aleks Terauds, W.P. Amy Liu, Steven L. Chown Contact: steven.chown@monash.edu ######################### Wet mass data for species ######################### File name: Macquarie_Collembola_Fresh_Mass.csv Weighed 50 live individuals per species (data in mg) using a Mettler Toledo microbalance (accurate to 1 ug) ################################### Critical thermal limits experiments ################################### F0 data File name: Macquarie_CTs_F0_V2.csv Acclimation Temperatures explanation 0 Static 0°C 5 Static 5°C 10 Static 10°C 15 Static 15°C 250 0°C with a temperature spike up to 25°C for 1 hour each day (with 30 min ramp up and down from this temperature) 525 5°C with a temperature spike up to 25°C for 1 hour each day (with 30 min ramp up and down from this temperature) 1025 10°C with a temperature spike up to 25°C for 1 hour each day (with 30 min ramp up and down from this temperature) 1525 15°C with a temperature spike up to 25°C for 1 hour each day (with 30 min ramp up and down from this temperature) 50 0°C with a temperature down to -5°C for 1 hour each day (with 30 min ramp down and up from this temperature) 55 5°C with a temperature down to -5°C for 1 hour each day (with 30 min ramp down and up from this temperature) 105 10°C with a temperature down to -5°C for 1 hour each day (with 30 min ramp down and up from this temperature) 155 15°C with a temperature down to -5°C for 1 hour each day (with 30 min ramp down and up from this temperature) Notes F0's acclimated at 10°C (rearing temp) were acclimated for 1-2 weeks prior to testing (with acclimation beginning directly after extraction in the lab - note that this might have been up to 1-2 weeks after collection due to transportation times) Other acclimations lasted for 7 days. The extra acclimations were only performed for species at F0 where I didn't think they would make it to F2 and there were enough individuals (Parisotoma and Folsomotoma) or where there were thousands (H. viatica) The temperature spike acclimations were only performed for H. viatica at F0, and these were done after springtails had been in the lab at 10C for 1-2 months. Temperature ramping rate for CTmax and CTmin = 0.05°C per minute Start temperature = 10°C for all experiments ######################### F2 data File name: Macquarie_CTs_F2.csv Acclimation Temperatures explanation 5 Static 5°C 10 Static 10°C 15 Static 15°C 1025 10°C with a temperature spike up to 25°C for 1 hour each day (with 30 min ramp up and down from this temperature) 105 10°C with a temperature spike down to -5°C for 1 hour each day (with 30 min ramp down and up from this temperature) Notes Acclimations lasted 7 days All conducted on F2 adults with the exception of P. insularis which were F3 adults Temperature ramping rate for CTmax and CTmin = 0.05°C per minute Start temperature = 10°C for all experiments ################################# Desiccation resistance experiment ################################# F0 data File name: Macquarie_Desiccation_F0.csv Species Species name Status Alien or indigenous Acclimation Temp Temperature (°C) at which springtails were acclimated for 1 week. Only some conditions done due to lack of numbers Test Temp Temperature (°C) the experiment was run at. Only some conditions done due to lack of numbers Replicate Pot Numbered 1-4 Individual Individual ID for springtail within a certain pot Time to death In minutes, checked every 10 minutes Date Date experiment was conducted Dry mass for group The combined weight (dry mass, mg) for the group of c. 10 individuals in each replicate pot Individual dry mass Group weight divided by the number of individuals ############################################# F2 data File name: Macquarie_Desiccation_F2_V2.csv Species Species name Status Alien or indigenous Acclimation Temp Temperature (°C) at which springtails were acclimated for 1 week. Test Temp Temperature (°C) the experiment was run at. Replicate Pot Numbered 1 - 4. Individual Individual ID for springtail within a certain pot Time to death In minutes, checked every 10 minutes Date Date experiment was conducted Dry mass for group The combined weight (dry mass, mg) for the group of c. 10 individuals in each replicate pot Individual dry mass All springtails in a single pot (~ 10 individuals) were weighed at once. In this column is the group weight divided by the number of individuals #################################################### Egg development rate and hatching success experiment #################################################### Methods Development Temperatures (°C) 0, 5, 10, 15, 20, 25, 30 - Eggs were transferred to each specific temperature within 1 day of laying (F2 parents at 10°C - eggs are F3 individuals) Light cycle 12 : 12 L : D CT Incubators/rooms Panasonic CT incubators (0, 5, 15, 25 & 30°C) and CT rooms (10 & 20°C) Sample size 5 replicate pots per temperature, with 10 eggs per replicate pot = 50 eggs per temperature for each species Endpoint 1. All eggs hatched; 2. Unhatched eggs are visibly dead; 3. Remaining eggs haven't hatched within 10 days (10 - 30°C) or 14 days (0 - 5°C) of last hatched egg within pot Data recorded Days to hatching (checked daily); hatching success (% hatched out of the 10 eggs within each pot) #################### Egg development rate #################### File name: Macquarie_DevelopmentTime_F2_slc_time.csv Species Species name Status Alien or indigenous Temperature Egg development temperature in °C Pot Number Replicate pot Egg number Individual egg (10 per replicate pot) Days to hatching Number of days until individual completely hatched from egg Rate 1/days ################ Hatching success ################ File name: Macquarie_DevelopmentTime_F2_slc_hatch_success.csv Species Species name Status Alien or indigenous Temperature Egg development temperature in °C Pot Number Replicate pot % Hatched % of individuals completely hatched out of egg within the replicate pot Alive Number of individuals that hatched Dead Number of individuals that did not hatch