Understanding the Cellular Internalisation of Antibody Therapeutics using Novel Sensors

Antibody targeted delivery systems like nanoparticles show promise in overcoming nucleic acid delivery challenges including degradation and cellular uptake. Optimising delivery systems requires understanding how antibody-targeting influences cellular interactions and nucleic acid delivery. Understanding these interactions can improve antibody selection for targeted delivery systems which is often based on affinity instead of internalisation. However, measuring cellular interactions requires improved tools to assess antibody-mediated internalisation and activity. In this thesis novel methods to assess antibody-mediated cellular interactions of targeted nucleic acid delivery systems were developed to understand how internalisation impacts delivery to assist selection of antibodies to maximise nucleic acid activity.