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Towards Understanding the Nuclear Organisation of Human Pluripotency Transcription Factors at High Spatial Resolution.

thesis
posted on 2024-06-09, 20:25 authored by ESTHER LOUISE MIRIKLIS
Human pluripotent stem cells are an important cell type during early development, possessing the ability to differentiate into the various cell types that compose the adult body. Establishment and maintenance of pluripotency is determined, in part, by transcription factor proteins. Transcription factors help to define cellular identity through their interactions with DNA and other nuclear proteins. However, the spatial arrangement of pluripotency transcription factors within the nucleus has thus far been underexplored. Using advanced fluorescence microscopy, the subcellular organisation of transcription factors was characterised, revealing that the nuclear organisation of key pluripotency transcription factors was distinct across different pluripotency states.

History

Principal supervisor

Jose Maria Polo

Additional supervisor 1

Toby D.M. Bell

Additional supervisor 2

Yu Bo Yang Sun

Year of Award

2024

Department, School or Centre

Australian Regenerative Medicine Institute (ARMI)

Campus location

Australia

Course

Doctor of Philosophy

Degree Type

DOCTORATE

Faculty

Faculty of Medicine, Nursing and Health Sciences