The design and development of lyotropic liquid crystalline lipidic nanoparticles in enhancing daptomycin’s antibacterial effect against methicillin-resistant Staphylococcus aureus

Increasing reports of daptomycin resistance in methicillin-resistant Staphylococcus aureus (MRSA), especially in the form of mprF and cls2 mutations pose a significant threat to human health. Therefore, it is important to improve the antibacterial efficacy of daptomycin and extend its lifespan as the last-line defense against multidrug-resistant (MDR) MRSA infections. In an attempt to resolve the daptomycin resistance crisis, this thesis designed and developed phytantriol-based cubosomes packaged with daptomycin to achieve improved daptomycin antibacterial efficacy. This thesis also attempted to develop phytantriol-based antimicrobial lipidic nanoparticles as a novel antibacterial agent that could be used alone or in combination with daptomycin The data from this thesis are valuable for the future treatment of Staphylococcus aureus infections.