Eric Mandela- PhD thesis_2020.pdf (8.15 MB)
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The architectural significance of Gram-negative periplasm

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posted on 2020-05-21, 09:49 authored by ERIC JOSEPH LOLEH MANDELA
This thesis investigates the architectural design principles of periplasmic space in bacterial cells. In this study, the periplasm was artificially increased in size using an artificial protein isoform, then a genetic screen was performed to identify periplasmic processes that rely on strict control of the periplasmic size. The strain with an enlarged periplasm was extensively characterised revealing few distinct phenotypes, an indicator that it adapts to the periplasmic size increase. How the strain adapts was investigated through transcriptional and proteomic profiling, where a dynamic adaptation response network was established giving insights into Gram-negatives ability to occupy different environmental niches.


Principal supervisor

Trevor Lithgow

Additional supervisor 1

Iain Hay

Year of Award


Department, School or Centre


Campus location



Doctor of Philosophy

Degree Type



Faculty of Medicine, Nursing and Health Sciences