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Systematic loss of functional screens identifies core-essential and pathway-specific functional circular RNAs
thesis
posted on 2024-08-15, 23:22 authored by MATTHEW TIMOTHY NEVECircular RNAs (circRNAs) are closed, single-stranded RNA molecules emerging as key players in cellular biology. Despite their importance, understanding their functions remains limited. We conducted high-throughput screens targeting circRNAs in cancer cells, revealing their roles in vital cellular processes and specific signaling pathways. By comparing different cellular contexts, we identified circRNAs essential for cell survival and those regulating specific pathways like WNT and MAPK. Validation studies highlighted circRERE(4-11) in ferroptosis regulation, circHUWE1(22,23) in apoptosis, circSMAD2(2-7) in WNT pathway, and circMTO1(2,RI,3) in MAPK signaling. This study provides insights into circRNA functions and offers a resource for future research in human cells.
