Structural characterization of thiolase and decarboxylase enzymes for biotechnological application in microbial degradation of water pollutants
thesisposted on 05.05.2020, 04:26 by SUKRITEE BHASKAR
The central theme of this thesis is to understand the microbial degradation of aromatic compounds. Two enzymes are selected for study, which belong to the central β-ketoadipate pathway, a key aromatic degradation pathway found in Pseudomonas Sp., Acinetobacter Sp. The first protein, β-keto acyl CoA thiolase (PcaF), catalyzes the last step of the β-ketoadipate pathway and the second protein of interest, γ-caboxymuconolactone decarboxylase (PcaC), belong to the protocatechuate branch of the β-ketoadipate pathway. These two proteins are located at two crucial point in the pathway, thus understanding these two catabolic enzymes will aid in development of better bioremediation designs.