Structural and functional characterisation of MACPF proteins in immunity

The human immune system consists of hundreds of thousands of nanoscopic molecular machines, referred to as proteins. This thesis explores a group called pore forming proteins, which self-assemble into ring structures in order to insert into and puncture cell barriers. These agents maintain human health by targeting and destroying rogue cells or invading pathogens. If left unchecked, however, the immune system can damage the body. This thesis visualised nanoscopic details of the architecture and organisation of these potent effectors. These findings provide an understanding of the function of these immune weapons and the underlying molecular mechanisms of certain immune diseases.