Post Translational Modifications and Interactome of STAT3

Signal Transducer and Activator of Transcription (STAT)3 is a member of the STAT family of latent transcription factors that relays signals from the extracellular environment to the nucleus. Whilst the activity of STAT3 is crucial for a plethora of biological functions, its dysregulated activity is implicated in many human tumours. However, the STAT3 activation mechanism and its biological consequences are far more complex than first thought. This thesis contributes to the current knowledge of STAT3 biology by investigating its non-genomic activities, post-translational modifications that regulate STAT3 activity and provides strategies for obtaining stable and pure full-length protein for structural studies.