Monash University
6127829_ThesisNgHonWei2018.pdf (1.48 MB)

Polymer modified ion exchange adsorbent for the flow-through chromatography of hepatitis B virus-like particle

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posted on 2018-04-15, 23:55 authored by HON WEI NG
Size selective flow-through chromatography (FC) was employed to adsorb impurities i.e. host cell proteins while large hepatitis B core antigen virus like particles (HB-VLPs) are recovered in the flow-through fraction. An inert polymer was grafted on adsorbents to improve the performance. The effect of feedstock conditions (size of target protein and protein load) and adsorbent design (adsorbent size, type of polymer chain and POEGMA architecture) on the performance of FC were investigated. Large size of HB-VLPs limits the protein load to 7 mg/ml, while optimal adsorbent design improved flow-through recovery by 5% compared to previous studies.


Campus location


Principal supervisor

Tey Beng Ti

Additional supervisor 1

Tang Siah Ying

Additional supervisor 2

Ooi Chien Wei

Year of Award


Department, School or Centre

Chemical & Biological Engineering

Additional Institution or Organisation

Chemical Engineering


Master of Engineering Science (Research)

Degree Type



Faculty of Engineering