Phage Display and Next-Generation Sequencing-Enabled Selection of Synthetic Nanobodies for Targeted Nanoparticle Delivery

This thesis focuses on developing and using nanobodies—small, single-domain antibody fragments—to improve how we find and study new biological tools for medicine. A large synthetic library of nanobodies was created to identify those that bind specifically to important disease-related targets by in vitro display. Advanced screening and sequencing methods were used to better understand how these nanobodies behave and how they might be used in research or therapy. The work also explores how nanobodies can help guide treatments to the right cells, improving the accuracy and potential of modern drug delivery systems.