Over the past few decades people have pursued using classical optical microscope to observe the world of nanoscience. Therefore, super resolution microscopy comes into being to allow imaging nano-scale systems under classical microscope in various ways, across the fields of chemistry, biology and physics. The work outlined in this thesis provides different tools for the sub-diffraction imaging of nano-scale objects, and the understanding of energy transfer can be expanded fundamentally, benefitting the fabrication of optoelectronics devices.