Novel engineering methods for the development of optogenetic tools
thesisposted on 2021-11-05, 01:10 authored by ALEXANDRA-MADELAINE TICHY
This thesis addresses two major challenges in the field of cellular optogenetics. It provides a streamlined method and plasmid repository for the engineering of light-activated protein-protein interactions, and introduces a conceptually novel design approach for light-activated G-protein coupled receptors based on structural information. This work facilitates the broader accessibility of optogenetics and presents significant improvements in activity and applicability of current optogenetic tools.