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Novel engineering methods for the development of optogenetic tools
thesisposted on 05.11.2021, 01:10 by ALEXANDRA-MADELAINE TICHY
This thesis addresses two major challenges in the field of cellular optogenetics. It provides a streamlined method and plasmid repository for the engineering of light-activated protein-protein interactions, and introduces a conceptually novel design approach for light-activated G-protein coupled receptors based on structural information. This work facilitates the broader accessibility of optogenetics and presents significant improvements in activity and applicability of current optogenetic tools.