NMR-based Structural and Dynamic Characterization of Bacterial Dsb Enzymes to support Fragment-Based Drug Design

Proteins are complex molecules that play many roles in living organisms. Understanding how proteins are structured, how they move, and how they interact with other molecules is crucial for drug design. In this work, Stefan used the technique of NMR to study the structure, motion, and interactions of two proteins that are crucial for the pathogenic bacteria B. pseudomallei and N. meningitidis. This work measured the dynamic behavior of these two proteins, providing crucial insights into their function, and identified small molecules that interact with them, providing much-needed starting points for the development of new drugs against these bacteria.