Monash University
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Microbial Hydrogen Cycling in the Human Gastrointestinal Tract

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posted on 2025-03-10, 03:27 authored by Caitlin Sarah Welsh
This thesis explores microbial hydrogen metabolism in the human gastrointestinal tract, focusing on the key pathways, enzymes and microorganisms involved, along with the influence of diet. By integrating cohort studies, -omics analyses, and culture-dependent experimental validation, this research identifies diverse hydrogenases and pathways involved in H₂ production and consumption. The previously uncharacterised group B [FeFe]-hydrogenase emerged as the most abundant and expressed hydrogenase throughout the microbiota, and was validated as active and responsible for H₂ production in diverse isolates, and dominantly Bacteroides. These findings further clarify how H₂ is cycled by microbiota, and offers new insights into gut health.

History

Principal supervisor

Chris Greening

Additional supervisor 1

Sam Forster

Additional supervisor 2

Rachael Lappan

Additional supervisor 3

Dena Lyras

Year of Award

2025

Department, School or Centre

Microbiology

Campus location

Australia

Course

Doctor of Philosophy

Degree Type

DOCTORATE

Faculty

Faculty of Medicine, Nursing and Health Sciences

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    Faculty of Medicine, Nursing and Health Sciences Theses

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