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Machine learning-based prediction of optimal fermentation conditions for soluble protein production in periplasm of Escherichia coli

thesis
posted on 04.01.2021, 03:10 by KULANDAI AROCKIA RAJESH PACKIAM
Maximizing the expression of recombinant proteins in E. coli has tremendous research interest. To date, studies have attempted optimizing factors relating to either gene expression (expression-level) or fermentation process (process-level) conditions to achieve high yields of RPP. However, understanding the combinatorial influence of expression and process-level factors is crucial for achieving the desired protein yields. This thesis demonstrates that a machine learning model based on expression and process levels can effectively predict the optimized fermentation conditions. The developed tool will enable researchers to predict optimal conditions for maximal recombinant protein production, reducing the time-consuming and expensive trial and error experiments.

History

Campus location

Malaysia

Principal supervisor

Ooi Chien Wei

Additional supervisor 1

Nagasundara Ramanan Ramakrishnan

Year of Award

2021

Department, School or Centre

School of Engineering (Monash University Malaysia)

Course

Doctor of Philosophy

Degree Type

DOCTORATE