Investigation of the molecular mechanisms driving human cytomegalovirus assembly and egress

More than half the world's population is infected with human cytomegalovirus (HCMV), increasing risks for the immuno-compromised, and causing significant congenital birth defects. The aim of this thesis was to expand the understanding of processes occurring during the late stages of HCMV replication. The viral proteins UL34 and UL49 were selected for comprehensive investigation, and late functions assigned using molecular virology methods. Additionally, using a proteomics-based approach, the cellular origin of the virion envelope was identified. This work contributes significantly to the understanding of the molecular processes governing HCMV infection and will inform the future development of novel antiviral strategies.