Large amount of food coming into the processing chain ends up as wastes, causing great negative economic and environmental implications. Winemaking waste is major food-loss stream, but is excellent source of beneficial dietary polyphenols, particularly polyphenol glucoside derivatives. This thesis identifies the valuable bioactive polyphenols in wine and wastes, establishes a model to evaluate winemaking process efficiency for polyphenols extraction, and demonstrates how winemaking wastes can be valorised into valuable products like bioactive polyphenols and prebiotic fibres. It not only represents a tremendous economic opportunity, but also an opportunity to improve the sustainability of the winemaking industry domestically and globally.