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Integrated approach for cell disruption and recovery of intracellular recombinant protein by using alcohol + salt based aqueous two-phase system
thesisposted on 28.08.2017, 00:18 by SEWN CEN LO
A simplified alcohol + salt ATPS based integrated strategy for the in situ production, release and recovery of intracellular recombinant EGFP from E. coli in a single unit operation was successfully developed. Overall, this study shows the efficiency of ATPS-based integrated process for the recovery of EGFP from whole broth culture of E. coli, as well as a feasible economic process for a commercial application.