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Integrated approach for cell disruption and recovery of intracellular recombinant protein by using alcohol + salt based aqueous two-phase system

thesis
posted on 28.08.2017, 00:18 by SEWN CEN LO
A simplified alcohol + salt ATPS based integrated strategy for the in situ production, release and recovery of intracellular recombinant EGFP from E. coli in a single unit operation was successfully developed. Overall, this study shows the efficiency of ATPS-based integrated process for the recovery of EGFP from whole broth culture of E. coli, as well as a feasible economic process for a commercial application.

History

Campus location

Malaysia

Principal supervisor

Ooi Chien Wei

Year of Award

2017

Department, School or Centre

School of Engineering (Monash University Malaysia)

Course

Doctor of Philosophy

Degree Type

DOCTORATE

Faculty

Faculty of Engineering

Exports

Keywords

Exports