Illuminating The Life of the Adenosine Receptors: Development and Application of Subtype-Selective Fluorescent Probes for the study of the human A1 and A2A Adenosine Receptors in Living Cells

Adenosine Receptors (AR) are membrane proteins which provide important therapeutic opportunities for many conditions including cardiovascular, inflammatory diseases, neuropathic pain, CNS deriders and cancer. However, translation of the AR ligands to the clinic has proved challenging and an improved understanding of AR pharmacology could promote the development of more efficacious therapies. This thesis describes the development and application of novel subtype-selective fluorescent probes with a focus to the A1 and A2A AR subtypes. These novel A1 and A2A fluorescent probes will be valuable pharmacological tools to the wider scientific community to aid in understanding of the molecular mechanisms underlying AR pharmacology in health and disease.