Identification of cancer specific endogenous HLA class I peptide repertoire in haematological and paediatric brain malignancy

Using a combination of high-resolution mass spectrometry (MS) flow cytometry, and other biochemical techniques the study investigates in-depth the immunopeptidome of AML including cell lines and clinical samples. the thesis also thoroughly investigates the immunopeptidome of DIPG, a type of rare and aggressive paediatric glioma. Through our optimised MS workflow, we have identified different peptides which have the potential of being translated at a clinical level for T cell mediated immunotherapy, hence fulfilling the unmet need for DIPG specific treatment.