Identification of anticancer proteins from Malaysian Bungarus fasciatus and Bungarus candidus venoms using brain tumour cells

<p>This study aims to purify and identify potential bioactive proteins exhibiting anticancer activities from Malaysian Bungarus fasciatus and Bungarus candidus venoms using human SH-SY5Y neuroblastoma and U-87MG glioblastoma cells. Bioassay-guided fractionation was performed on both venoms using size-exclusion and reverse-phase chromatography. Neurotoxin-3FTX-LK and B-BuTX-A-AL2 were isolated from BF and BC venoms, respectively, and tested for anticancer activities. Ntx-3FTX-LK and B-BuTX-A-AL2 had demonstrated cytotoxicity and antiproliferative effects in concentration- and time-dependent manner, and induced caspase-3 time-dependent apoptosis in both cells. Protein expression profiles and brain tumour-related pathways revealed the association in cellular-metabolic process, transcriptional-translational protein synthesis, apoptosis, and immune response signaling.</p>