High resolution 3D imaging and analysis of single cells

Single-cells-based studies are essential in disentangling cellular heterogeneity, benefiting both the treatment and prevention of diseases. All the new findings in single-cells-based studies can hardly be obtained without the advances in microscopy. This thesis focuses on the development of new microscopic approaches applicable to the interpretation and analysis of the behaviours of single bacterial cells, in order to provide unique assessments on the effectiveness of new antibiotics during drug uptake.