Monash University

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Reason: Under embargo until March 2018. After this date a copy can be supplied under Section 51 (2) of the Australian Copyright Act 1968 by submitting a document delivery request through your library

Functional roles of the Caenorhabditis elegans Y-box binding protein, CEY-3 in post-transcriptional gene regulation during development

posted on 2017-02-13, 23:14 authored by Md Masuder Rahman
In many tissues, post-transcriptional gene regulation (PTGR) is a particularly important mechanism for regulating gene expression. Binding of RNA to RNA-binding proteins (RBPs) is key to controlling mRNA localization, translation and turnover. We have been using the Caenorhabditis elegans model system to study a conserved family of C. elegans cold-shock (Y-box) RNA-binding proteins (CEYs). CEYs are expressed in the germlines but unexpectedly, we identified that C. elegans lacking CEY-2 is sensitive to heat stress, and that the proteins CEY-2 and CEY-3 are localized to some neurons upon exposure to elevated temperatures. This data suggests that the C. elegans Y-box family of proteins have broad functions including during heat stress condition.
   CEYs are also required for the integrity and stability of the P granules. Loss of CEY-2 or CEY-3 results in a significant reduction in progeny number and they function redundantly to reduce brood size and increase embryonic lethality. CEY-3 is predominantly express in the gonads where it localizes to RNA-rich perinuclear cytoplasmic granules (P granules). Moreover, we identified the protein interactors of CEY-3 by mass spectrometry-based immunoprecipitation proteomics. Interestingly two of the interactors, non-muscle myosin-2 (NMY-2) and actin (ACT-2) known as actin cytoskeleton, were found to be required for localization of P granule components including PGL-1, IFET-1, CAR-1, CEY-2 and CEY-3 into oocytes. This suggests that CEY-3 may function as adapter protein to connect between the P granules and NMY-2 for the localization of P granule components to the site of translation.


Principal supervisor

Peter R. Boag

Year of Award


Department, School or Centre

Biomedical Sciences (Monash Biomedicine Discovery Institute)

Additional Institution or Organisation

Biochemistry and Molecular Biology

Campus location



Doctor of Philosophy

Degree Type



Faculty of Medicine Nursing and Health Sciences