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Functional roles of the Caenorhabditis elegans Y-box binding protein, CEY-3 in post-transcriptional gene regulation during development

thesis
posted on 13.02.2017, 23:14 authored by Md Masuder Rahman
In many tissues, post-transcriptional gene regulation (PTGR) is a particularly important mechanism for regulating gene expression. Binding of RNA to RNA-binding proteins (RBPs) is key to controlling mRNA localization, translation and turnover. We have been using the Caenorhabditis elegans model system to study a conserved family of C. elegans cold-shock (Y-box) RNA-binding proteins (CEYs). CEYs are expressed in the germlines but unexpectedly, we identified that C. elegans lacking CEY-2 is sensitive to heat stress, and that the proteins CEY-2 and CEY-3 are localized to some neurons upon exposure to elevated temperatures. This data suggests that the C. elegans Y-box family of proteins have broad functions including during heat stress condition.
   
   CEYs are also required for the integrity and stability of the P granules. Loss of CEY-2 or CEY-3 results in a significant reduction in progeny number and they function redundantly to reduce brood size and increase embryonic lethality. CEY-3 is predominantly express in the gonads where it localizes to RNA-rich perinuclear cytoplasmic granules (P granules). Moreover, we identified the protein interactors of CEY-3 by mass spectrometry-based immunoprecipitation proteomics. Interestingly two of the interactors, non-muscle myosin-2 (NMY-2) and actin (ACT-2) known as actin cytoskeleton, were found to be required for localization of P granule components including PGL-1, IFET-1, CAR-1, CEY-2 and CEY-3 into oocytes. This suggests that CEY-3 may function as adapter protein to connect between the P granules and NMY-2 for the localization of P granule components to the site of translation.

History

Principal supervisor

Peter R. Boag

Year of Award

2017

Department, School or Centre

Biochemistry and Molecular Biology

Campus location

Australia

Course

Doctor of Philosophy

Degree Type

DOCTORATE

Faculty

Faculty of Medicine Nursing and Health Sciences