Exploring the Potential of High-Frequency Ultrasound as a Means for Modulating Cell Behaviour

This thesis demonstrates the use of high-frequency ultrasound as a non-invasive, biocompatible method to modulate cell behaviour in sperm and mesenchymal stromal cells (MSCs). A droplet-based acoustofluidic platform restored motility in 34% of immotile sperm and maintained it with repeated pulses, reducing motility loss by 30%. Ultrasound also enhanced MSC morphology, increasing cell area by 30% and metabolic activity by 58%, without compromising viability. These results establish ultrasound as a scalable, drug-free alternative for cell activation in assisted reproduction and regenerative medicine, highlighting its potential for future clinical and mechanobiological applications.