Engineering multifunctional biomolecules for imaging and therapeutic applications

Biomolecules with multiple functions have potential important basic research, diagnostic, and therapeutic applications. Such molecules are generally synthesized using either particulate nanochemistry or protein engineering approaches. The study described in this thesis engineered biomolecules with multiple functions for use in light microscopy imaging and therapeutic applications. The methods were based on an enzyme-assisted chemical reaction and genetic fusion of proteins onto molecular scaffolds. Overall, the platforms developed in this thesis may extend frontiers in novel nanotechnologies with important future research and health applications.