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Reason: Under embargo until November 2025. After this date a copy can be supplied under Section 51(2) of the Australian Copyright Act 1968 by submitting a document delivery request through your library.
Engineering cytosolic transport for improved therapeutic delivery
thesisposted on 2022-11-03, 12:29 authored by Joshua Rennick
Biopharmaceuticals have transformed modern medicine by enabling unprecedented biological control over disease beyond the capabilities of small molecule drugs. Biological drug applications are highly diverse and represent most of the top-selling drugs worldwide. However, inefficient drug delivery to the interior of cells has limited development for intracellular applications. This poses a substantial area to expand the possibilities of biopharmaceuticals. This thesis develops sensors to quantify intracellular pH and cytosolic delivery efficiency as well as a directed evolution methodology to discover more efficient endosomal escape peptides. Together, these tools may enable more effective intracellular biopharmaceutical delivery to improve human health.