Monash University

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Reason: Under embargo until June 2018. After this date a copy can be supplied under Section 51 (2) of the Australian Copyright Act 1968 by submitting a document delivery request through your library

Development of cell wall model of Escherichia coli for periplasmic protein release and purification of interferon-α2b using aqueous two-phase system

posted on 2017-06-13, 04:17 authored by RAMALAKSHMI SUBBARAYALU
Osmotic shock is the most reliable method for periplasmic protein recovery. Osmotic shock relies on the intactness of cell wall which is modified by the changes in fermentation conditions. Fermentation conditions alter the cell wall property which in turn affects osmotic shock for periplasmic protein release. Osmotic shock uses large amount of sucrose yet, recycling option has not been considered. In order to address these issues, this study linked the changes in fermentation conditions with the periplasmic protein release. Sucrose from osmotic shock was reused to purify the recovered proteins and hence this served as a sustainable downstream process.


Campus location


Principal supervisor

Ramanan Ramakrishnan

Year of Award


Department, School or Centre

School of Engineering (Monash University Malaysia)


Doctor of Philosophy

Degree Type



Faculty of Engineering