Monash University
4712500_monash_163872.pdf (14.14 MB)

Dendritic cell regulation of HIV-1 latency in CD4+ T-cell

Download (14.14 MB)
posted on 2017-03-02, 03:54 authored by Kumar, Nitasha Asmita
Combination antiretroviral therapy (cART) is able to control HIV-1 replication and prevent acquired immune deficiency syndrome (AIDS), however does not provide a cure. The major barrier to HIV-1 cure is the persistence of long-lived, latently infected resting CD4+ T-cells. We developed an in vitro dendritic cell (DC)-T-cell latency model, which showed that myeloid (m)DC induced post-integration latent infection in non-proliferating, resting and proliferating CD4+ T-cells. Gene expression profiles indicated that mDC could regulate the establishment of latent infection in non-proliferating and proliferating CD4+ T-cells through interaction between adhesion molecules, co-stimulatory molecules and immune checkpoints. On the contrary, pDC-T-cell interactions led to the inhibition of latent infection in co-cultured resting CD4+ T-cells and death of proliferated CD4+ T-cells. Gene expression profiles suggested that inhibition is mediated through type-I and III interferons. Investigation of the mechanisms of mDC mediated establishment of latent infection and pDC mediated inhibition of latent infection, may provide novel elimination strategies for HIV-1 cure.


Principal supervisor

Sharon R Lewin

Additional supervisor 1

Paul U Cameron

Additional supervisor 2

Vanessa A Evans

Year of Award


Department, School or Centre

Infectious Disease

Campus location


Degree Type



Faculty of Medicine Nursing and Health Sciences

Usage metrics

    Faculty of Medicine, Nursing and Health Sciences Theses


    No categories selected