Cryo-EM grid preparation via SAW atomisation

Cryo-Electron Microscopy (Cryo-EM) is a powerful tool to image the structure of proteins embedded in a layer of ice typically less than 100 nm thick, and as it sounds, the sample preparation is challenging. In this thesis, we proposed using a novel acoustic atomiser (SAW atomiser) to deposit aqueous samples onto the Electron Microscopy grid which acts as a support for thin ice. We showed that the method is functional and then characterised the atomiser and the grid to enhance the sample preparation process. This approach creates opportunities for time-resolved studies of protein reactions.