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Cloning, expression and characterization of xylanases from Paenibacillus sp. isolated from landfill leachate

thesis
posted on 10.12.2017, 22:39 authored by TZE CHIEN PATRIC CHUA
In landfills, buried plant biomass are often degraded by soil microorganisms using enzymes. These enzymes, collectively termed as lignocellulases, can be useful tools to improve various processes in the industry. Although the application of these enzymes has been practiced, there are always discoveries of new enzyme strains with undescribed properties from nature. In particular, the specific adaptations to the landfill environment (e.g. wide pH fluctuations, substrate scarcity) remains largely unknown. This project describes the isolation of lignocellulose-degrading bacteria from a local landfill site, its subsequent genome sequencing, gene cloning and characterization of the attributes of the produced enzymes.

History

Campus location

Malaysia

Principal supervisor

Sui Mae Lee

Year of Award

2017

Department, School or Centre

School of Sciences (Monash University Malaysia)

Course

Doctor of Philosophy

Degree Type

DOCTORATE

Faculty

Faculty of Science