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Characterisation of the Pleurotolysin MACPF Pore Assembly

thesis
posted on 09.10.2019, 04:32 by STEPHANIE CATHERINE KONDOS
MACPF pore-forming proteins 'punch holes' in cell membranes, as part of their role in immune defence or microbial invasion. However, due to a small number of pore structures, our understanding of how these proteins form pores is incomplete. In this PhD, the structural characterisation of pleurotolysin, a MACPF from the oyster mushroom, was conducted. Structures were determined for pleurotolysin as a single subunit and as an assembled, multi-subunit pore complex. Furthermore, structures were obtained of non-inserted complexes, 'trapped' by molecular pins that tethered the regions responsible for pore formation in place. Together, these structures form snap-shots of how MACPF proteins form pores.

History

Principal supervisor

Michelle Anne Dunstone

Year of Award

2019

Department, School or Centre

Biochemistry and Molecular Biology

Course

Doctor of Philosophy

Degree Type

DOCTORATE

Faculty

Faculty of Medicine, Nursing and Health Sciences

Exports

Exports