Monash University

Embargoed and Restricted Access

Reason: Under embargo until 30 May 2023. After this date a copy can be supplied under Section 51(2) of the Australian Copyright Act 1968 by submitting a document delivery request through your library

Bioengineered 3D microenvironments for in vitro T cell generation and CAR-T cell delivery

posted on 2022-08-23, 04:39 authored by ANISHA BALACHANDRAN SURAIYA
T cells are an essential arm of the adaptive immune system and are also harnessed as therapeutic agents for cancer immunotherapy. This thesis has focussed on the development of bioengineered 3-dimensional (3D) hydrogel microenvironments to address some of the unmet clinical needs related to T cells. This study demonstrates the suitability of hydrogel microstructures (microgels) as 3D culture platforms for in vitro generation of T cells with upscaling potential and for thymic epithelial cell maintenance. This research also investigates the feasibility of microgels as delivery vehicles of chimeric antigen receptor (CAR)-T cells for augmenting solid tumour immunotherapy.


Campus location


Principal supervisor

John Stanley Forsythe

Additional supervisor 1

Ann Chidgey

Additional supervisor 2

Nicholas Boyd

Additional supervisor 3

Richard Boyd

Year of Award


Department, School or Centre

Materials Science and Engineering


Doctor of Philosophy

Degree Type



Faculty of Engineering