Acoustofluidic Technologies for Single Cell Analysis

Microfluidic and lab-on-a-chip technologies allow precise manipulation of small fluid volumes and cells, a need in many biomedical applications. Surface acoustic waves (SAWs) offer a non-contact, biocompatible method for on-chip cell control. This research integrates SAW devices with light-sheet fluorescent microscopy (LSFM) for high-resolution 3D imaging of single cells. The developed acousto-optofluidic device enables controlled imaging of white blood cells ingesting bacteria in a semi-automated manner. This dissertation demonstrates the potential of SAW devices for single cell control and their compatibility with LSFM, opening new avenues for on-chip manipulation and cell analysis in diverse research fields.