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A proteomics and bioinformatics study of peptides in the human immunopeptidome

thesis
posted on 19.12.2020, 04:57 by SHUTAO MEI
The presentation of protein degraded peptides by human leukocyte antigen class I (HLA-I) molecules forms the basis of the CD8+ T cell mediated immune response. However, current knowledge on how factors can determine peptide’s immunogenicity remains to be explored. This thesis has focused on two significant factors post-translational modification (PTM) as well as binding between peptide and HLA-I. The outcome has revealed that a predominant of HLA-I bound peptides bearing asparagine deamidation, one type of PTM, is associated with N-glycosylated precursors and potentially being utilized by the immune system for monitoring aberrant glycosylation. Additionally, tools for the prediction ofHLA-I peptide binding has been extensively reviewed and evaluated for guiding researchers to select most suitable predictors. Finally, a new tool with the model customisation function has been developed to solve the current limitation and to facilitate the development of immunotherapy.

History

Principal supervisor

Anthony Purcell

Additional supervisor 1

Jiangning Song

Additional supervisor 2

Nathan Croft

Year of Award

2020

Department, School or Centre

Biochemistry and Molecular Biology

Additional Institution or Organisation

Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute

Campus location

Australia

Course

Doctor of Philosophy

Degree Type

DOCTORATE

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