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monash2745Expression system for clostridium species.pdf (535.11 kB)

Expression system for clostridium species

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posted on 2016-09-07, 06:09 authored by Eric A. Johnson, Marite Bradshaw, Julian I. Rood, Dena Lyras
A system is used to express clostridial gene constructions in a clostridial host. A mobilizable transfer plasmid is described which permits the direct transfer of the plasmid, and genes carried on it, from E. coli into Clostridium species. A promoter is described for use in clostridial species. Also, a useful host strain is used which is nontoxigenic and which permits high levels of expression of clostridial genes using the clostridial promoter.

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