RNA Scope Chromogenic - Analysis with Tolerence Check (BX60) - Manual Threshold.ijm (10.51 kB)
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RNA Scope Chromogenic - Analysis with Tolerence Check (BX60) - Manual Threshold.ijm

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posted on 15.05.2020, 05:21 by Felix BennettsFelix Bennetts, Cameron NowellCameron Nowell
Script used to analyse chromogenic RNA scope using tolerance check and a manual threshold. Outputs two images images with manual tolerance check for each signal (blue and red) and counted as dots per signal. Outputs a composite image combining the two tolerance images and restricted to manual threshold selected by user. Results output as a table include threshold values selected, nuclei area, number of dots and number of dots per 10000 pixels of nuclear area.

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