Live Cell Analysis (3 and 4 Channel)

Fiji/ImageJ macro for analysis of live cell imaging data of mouse B cells captured on an Image Express Micro.

Macro was developed by Cameron J Nowell of Monash University (cameron.nowell@monash.edu) in collaboration with Ben Croker (bcroker@ucsd.edu).

Code will take raw images in individual time point folders and combine them into hyperstacks for analysis. Analysis will count each cell type (CellTracker, PI and AnnexinV) and determine the percentage ammount of each. Percentages of double and triple positive populations are also determined. The analysis is designed so that any given cell is only ever counted and classified once.