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Junction Analysis

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posted on 2022-04-28, 06:12 authored by Cameron NowellCameron Nowell

Fiji/ImageJ macro for analysis cell tight junctions using TiJOR analys adapted from Terryn et al (2013) Cytometry Part A


Macro was developed by Cameron J Nowell of Monash University (cameron.nowell@monash.edu) in collaboration with Ami Mehta (ami.mehta@monash.edu).


Code will take Leica LIF files Analysis will extract the junction channel (assumed to be channel 3) and analyse 

the TiJOR values


Requirements - Standard install of Fiji 

Assumptions 

 - Data is in Leica LIF format

 - Data is 3 channel

 - Junction stain (e.g. VE-cadherin) is in the 3rd channel

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