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Recombinant mouse CRISP protein add-back experiments

I. Representative sperm from Crisp1, and Crisp4 wild type and knock out mice before and after exposure to 1 uM recombinant mouse CRISP proteins.

II. Representative sperm from Crisp1 knockout sperm before and after exposure to 1 uM recombinant mouse CRISP4 protein and vice versa.

Sperm were tethered to glass slides and recorded using an Olympus AX-70 upright microscope equipped with ORCA-Flash4.0 v2 sCMOS camera. Frames were acquired at 400 fps.

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