Monash University
4.CTRa merge 9E10 sCT8-32.avi (1.17 MB)

Internalisation of sCT(8-32)-AF568 and 9E10-AF647 in COS-7 cells stably expressing the hCTRaLeu

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posted on 2017-09-20, 05:36 authored by Emma Dal MasoEmma Dal Maso, Sebastian FurnessSebastian Furness, PATRICK SEXTON
Confocal field images of COS-7 stably expressing hCTRaLeu were incubated with 1 μM sCT(8-32)-AF568 and 1 µg/ml anti-cMyc antibody (9E10-AF647) on ice. Cells were extensively washed. Imaged were acquired every 1 min for 60 min at 37 ̊C. Images are presented as maximum intensity projections for 9E10-AF647 emission (green), sCT-(8-32)-AF568 (red) emission. Representative images of 2 independent experiments.