Internalisation of sCT(1-32)-ROX and 9E10-AF647 in COS-7 cells stably expressing the hCTRaLeu.
mediaposted on 20.09.2017, 04:52 by Emma Dal MasoEmma Dal Maso, Sebastian FurnessSebastian Furness, PATRICK SEXTONPATRICK SEXTON
Confocal field images of COS-7 stably expressing hCTRaLeu were incubated with 1 μM sCT-ROX and 1 µg/ml anti-cMyc antibody (9E10-AF647) on ice. Cells were extensively washed. Imaged were acquired every 1 min for 60 min at 37 ̊C. Images are presented as maximum intensity projections for 9E10-AF647 emission (green), sCT-ROX (red) emission. Representative images of 2 independent experiments.