4.CTRa merge 9E10 sCT1-32.avi (1.83 MB)

Internalisation of sCT(1-32)-ROX and 9E10-AF647 in COS-7 cells stably expressing the hCTRaLeu.

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posted on 20.09.2017, 04:52 by Emma Dal Maso, Sebastian Furness, PATRICK SEXTON
Confocal field images of COS-7 stably expressing hCTRaLeu were incubated with 1 μM sCT-ROX and 1 µg/ml anti-cMyc antibody (9E10-AF647) on ice. Cells were extensively washed. Imaged were acquired every 1 min for 60 min at 37 ̊C. Images are presented as maximum intensity projections for 9E10-AF647 emission (green), sCT-ROX (red) emission. Representative images of 2 independent experiments.