Confocal field images of COS-7 stably expressing
hCTRaLeu were incubated with 1 µg/ml
anti-cMyc
antibody (9E10-AF647) on ice. Cells were extensively washed. Imaged
were acquired every 1 min for 60 min at 37 ̊C. Images are presented as maximum intensity
projections for 9E10-AF647 emission Representative images of 2 independent experiments.