The file contains raw movies of head tethered sperm from CRISP1, CRISP2, CRISP4 and CRISP1/4 wild type and knock out mouse lines. Sperm images were recorded at 400 fps using an Olympus AX-70 upright microscope equipped with ORCA-Flash4.0 v2 sCMOS camera. N = 25-30 sperm/genotype.