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mirrorCheck results for 4 public datasets

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posted on 2025-03-27, 05:09 authored by Katherine ScullKatherine Scull
<p dir="ltr">Each zipped folder contains results files from reanalysis of public data in our publication, "mirrorCheck: an R package facilitating informed use of DESeq2’s lfcShrink() function for differential gene expression analysis of clinical samples" (see also the Collection description).</p><p dir="ltr">These files were produced by rendering the Quarto documents provided in the supplementary data with the publication (one per dataset). The Quarto codes for the 3 main analyses (COVID, BRCA and Cell line datasets) performed differential gene expression (DGE) analysis using both DESeq2 with lfcShrink() via our R package mirrorCheck, and also edgeR. Each zipped folder here contains 2 folders, one for each DGE analysis. Since DESeq2 was run on data without prior data cleaning, with prefiltering or after Surrogate Variable Analysis, the 'mirrorCheck output' folders themselves contain 3 sub-folders titled 'DESeq_noclean', 'DESeq_prefilt' and 'DESeq_sva". The COVID dataset also has a folder with results from Gene Set Enrichment Analysis. Finally, the fourth folder contains results from a tutorial/vignette-style supplementary file using the Bioconductor "parathyroidSE" dataset. This analysis only utilised DESeq2, with both data cleaning methods and testing two different design formulae, resulting in 5 sub-folders in the zipped folder.</p>

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