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Raw mass spec data of PRC2-PALI1PIR-long complex in the presence or absence of SAM

posted on 09.06.2021, 04:30 by Qi Zhang
For the identification of methylations in the recombinant PRC2-PALI1PIR-long complex, 0.5 µM protein was incubated in the presence or absence of 20 µM SAM (NEB #B9003) in 25 mM HEPES pH 8.0, 50 mM NaCl, 2 mM MgCl2, 2 mM 2-mercaptoethanol and 10 % glycerol for 1 hour at 30 °C, in a total final volume of 85 µL. The proteins were then alkylated, subjected to tryptic digestion and the tryptic peptides where treated and analysed by MS/MS.