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Negative Stain Transmission Electron Microscopy data for tutorials.

dataset
posted on 2025-11-10, 23:10 authored by Matthew BelousoffMatthew Belousoff
<p dir="ltr">Subset of the Negative stain dataset from:</p><p dir="ltr">Chen, M.Z., Yuen, D., McLeod, V.M. <i>et al.</i> A versatile antibody capture system drives specific in vivo delivery of mRNA-loaded lipid nanoparticles. <i>Nat. Nanotechnol.</i> <b>20</b>, 1273–1284 (2025). https://doi.org/10.1038/s41565-025-01954-9</p><p dir="ltr">Used as training material to train students on how to analyze negative stain TEM datasets. </p><p dir="ltr">Collected on Thermo L120C Microscopy fitted with a Ceta direct electron detector.</p><p dir="ltr">Acceleration Voltage: 120 kV</p><p dir="ltr">Physical Pixel Size: 1.55 Angstrom/pix</p><p dir="ltr">Spherical Abberation: 2.7 mm</p><p dir="ltr">Data encoded in FEI SER format.</p>

Funding

ARC Training Centre for Cryo-Electron Microscopy of Membrane Proteins for Drug Discovery

Australian Research Council

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